anti- DGCR8 C-Terminal antibody

Product Description

anti- DGCR8 C-Terminal antibody is available at Gentaur for Next week Delivery.

Purification: Immunogen affinity purified

Background: Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA(miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts(pri-miRNAs) to release precursor miRNA(pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs(PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer(of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding(PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine(m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing(PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal(By similarity)..

Immunogen: DiGeorge syndrome critical region gene 8

Synonyms: C22orf12, DGCR8, DGCRK6, Gy1

Reactivity: Human, Mouse, Rat

Tested Application: ELISA, WB, IHC, IF, IP, FC

Recommended dilution: WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200

Image 1: Immunohistochemistry of paraffin-embedded human breast cancer using FNab02351(DGCR8 antibody) at dilution of 1:100

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Image 4: HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab02351(DGCR8 antibody) at dilution of 1:1000

Gene ID: 54487

Research Area: Metabolism

Uniprot ID: Q8WYQ5