Macs Beads

Posted by Lieven Gevaert, Gentaur BV on 14th May 2021

Magnetic beads for cell separation

Custom camel or camelid antibodies, nanobodies labeling with small beads for positive selection of 50 nm.

All kind of tags can be added to you camle antibody. FLAG, GFP, HA.

The beads and the fc will be integrated in your sell and expulsed by exocytosis.

Except for CD11 monocytes that will be activated by the positive selection.

CD11 cannot be selected by positive magnetic selection to be put back in culture.

T cells, NK cell, B cells can be selected by the very small 50 nm beads. These beads are so small that since the first use of positive selection by Stephen Miltenyi in 1996 it would have been known if they prevent reculturing lymphocytes.

However the cell state could be slightly changed by positive magnetic selection, like for monocytes CD11.

Thats why we use 250 nm beads for negative selection. The 250 nm beads are much more preformant in taking out cells. As sales representative for Miltenyi we expetiences a lot of times in the laboratory of Professor Goldman in ULB Erasme that CD34 positive selection for reculture and reinjection in lymphocyte depleted patients was a perfect solution. This was before the use of the Clinimacs. Especially in 1998 in Liege Sart Tilman this was a much used protocol and we can now tell that these labs where the precursers of CD34 selection for in vivo therapeutic use.

Lieven Gevaert, Bio-ir. Gent 1996, Scientific Director Gentaur BV, Brussels

Vaslilyi Abaev, Ms. Biotechnology, Sofia 2009, Scientific custom conjugation Manager Gentaur LTd. London

Camel beads are easlily biotinilated and thant couple to streptavidin magnetic beads

ID HUMAN CELL SURFACE MOLECULES Catalog # Size/Volume

Price
Euro
Catalog # Size/ Volume Price

 Euro

1 cd 2 ± T Cells cd2 250 250 nm 2 ml 350 cd2 50 50 nm 2 ml 450
2 cd 3 ± T Cells cd3 250 250 nm 2 ml 350 cd3 50 50 nm 2 ml 450
3 cd 4 ± T Helper Cells cd4 250 250 nm 2 ml 350 cd4 50 50 nm 2 ml 450
4 cd 5 ± Thymocytes cd5 250 250 nm 2 ml 375 cd5 50 50 nm 2 ml 475
5 cd 6 ± Mature T Cells cd6 250 250 nm 2 ml 375 cd6 50 50 nm 2 ml 475
6 cd 7 Thymocytes,T/pre B,NK cd7 250 250 nm 2 ml 375 cd7 50 50 nm 2 ml 475
7 cd 8 ± T Cells cd8 250 250 nm 2 ml 350 cd8 50 50 nm 2 ml 450
8 cd 9 platelets, pre B,mono,endothelia,epithilia cd9 250 250 nm 2 ml 375 cd9 50 50 nm 2 ml 475
9 cd 10 ± Granulocytes cd10 250 250 nm 2 ml 375 cd10 50 50 nm 2 ml 475
10 cd 11a/LFA 1 Leukocytes cd11a 250 250 nm 2 ml 375 cd11a 50 50 nm 2 ml 475
11 cd 11b ± mono -Granulocytes cd11b 250 250 nm 2 ml 385 cd11b 50 50 nm 2 ml 500
11c cd 11c NK Cells, Monocytes cd11c 250 250 nm 2 ml 375 cd11c 50 50 nm 2 ml 475
12 cd 13 ± gran, mono,mast and GM progenitors cd13 250 250 nm 2 ml 375 cd13 50 50 nm 2 ml 475
13 cd 14 ± mono cd14 250 250 nm 2 ml 385 cd14 50 50 nm 2 ml 500
14 cd 15 ± Granulocytes cd15 250 250 nm 2 ml 410 cd15 50 50 nm 2 ml 500
15 cd 16 ± NK cells cd16 250 250 nm 2 ml 375 cd16 50 50 nm 2 ml 475
16 cd 18 ± monocytes, lymphocytes cd18 250 250 nm 2 ml 375 cd18 50 50 nm 2 ml 475
17 cd B4419 dendritic cells, B cells cd19 250 250 nm 2 ml 395 cd19 50 50 nm 2 ml 475
18 cd 20 pre,resting and activated B Cells cd20 250 250 nm 2 ml 375 cd20 50 50 nm 2 ml 475
19 cd 21 ± B Cells dendritic and some epithelial cd21 250 250 nm 2 ml 375 cd21 50 50 nm 2 ml 475
20 cd 22 ± B Cells cd22 250 250 nm 2 ml 395 cd22 50 50 nm 2 ml 475
21 cd 23 ± mature B cells cd23 250 250 nm 2 ml 350 cd23 50 50 nm 2 ml 450
22 cd 25 ± Activated T/B cells cd25 250 250 nm 2 ml 375 cd25 50 50 nm 2 ml 475
23 cd 27 ± Thymocytes, Mature T lymphocytes cd27 250 250 nm 2 ml 375 cd27 50 50 nm 2 ml 475
24 cd 31 plate, mono, gran,lympho,endothelial cd31 250 250 nm 2 ml 375 cd31 50 50 nm 2 ml 475
25 cd 33 ± myeloid cells cd33 250 250 nm 2 ml 395 cd33 50 50 nm 2 ml 475
26 cd 34 Progenitor cells cd34 250 250 nm 2 ml 395 cd34 50 50 nm 2 ml 475
27 cd 45 all human leukocytes cd45 250 250 nm 2 ml 395 cd45 50 50 nm 2 ml 475
28 cd 45 RO ± T Cells cd45ro250 250 nm 2 ml 395 cd45ro50 50 nm 2 ml 475
29 cd 45 RA ± T, B, NK cells cd45ra250 250 nm 2 ml 395 cd45ra50 50 nm 2 ml 475
30 HLA-DR ± antigen Presenting Cells hla250 250 nm 2 ml 385 hladr50 50 nm 2 ml 450
31 cd 56 ± NK Cells cd56 250 250 nm 2 ml 395 cd56 50 50 nm 2 ml 450
ID HUMAN CELL SURFACE MOLECULES Catalog # Volume Price Catalog # /Volume Price
32 cd 62E Selectin endotheleum cd62e 250 250 nm 2 ml 395 cd62e 50 nm 2 ml 450
33 cd 62 L Selectin Neutrophils, monocytes,T,B cd62l 250 250 nm 2 ml 395 cd62l50 50 nm 2 ml 475
34 cd 62 P Selectin cd62p250 250 nm 2 ml 395 cd62p50 50 nm 2 ml 475
35 cd 71 Transferrin Receptor cd71 250 250 nm 2 ml 450 cd71 50 50 nm 2 ml 475
36 cd 90 [Thy-1] early cd34 and mature cd34 cd90 250 250 nm 2 ml 475 cd90 50 50 nm 2 ml 575
37 cd 114 G-CSFR neutrophils,myeloid,endothel cd114 250 250 nm 2 ml 475 cd114 50 50 nm 2 ml 575
38 cd 116 GM-CSFalpha, non blocking cd116 250 250 nm 2 ml 475 cd116 50 50 nm 2 ml 575
39 cd 116 GM-CSFalpha,blocking cd116 250 250 nm 2 ml 475 cd116b50 50 nm 2 ml 575
40 cd 121a IL-1R non blocking cd121a250 250 nm 2 ml 475 cd121a50 50 nm 2 ml 575
41 cd 121a IL-1R blocking cd121ab250 250 nm 2 ml 475 cd121ab50 50 nm 2 ml 575
42 cd 128 [IL-8RA] mono, gran cd128 250 250 nm 2 ml 475 cd12850 50 nm 2 ml 575
43 cd 130 [gp 130] T,B cd130 250 250 nm 2 ml 375 cd130 50 50 nm 2 ml 450
44 cd 152 [CTLA-4] cd152 250 250 nm 2 ml 475 cd152 50 nm 2 ml 575
45 cd 154 [CD40L] Activated T cd154 250 250 nm 2 ml 475 cd154 50 50 nm 2 ml 575
45a Anti Caveolin cav250 250 nm 2 ml 550 cav50 50 nm 2 ml 650
45b Epithelial Specific Antigen esa250 250 nm 2 ml 400 esa50 50 nm 2 ml 475
MOUSE CELL SURFACE MOLECULES
46 cd 4 ± T cells cd4m250 250 nm 2 ml 395 cd4m50 50 nm 2 ml 495
47 cd 5 ± T,B cells cd5m250 250 nm 2 ml 395 cd5m50 50 nm 2 ml 495
48 cd 8a T cells cd8am250 250 nm 2 ml 395 cd8am50 50 nm 2 ml 495
49 cd 11b ± Macrophages/Monocytes cd11bm250 250 nm 2 ml 395 cd11bm50 50 nm 2 ml 495
50 cd 19 ± B cells cd19m250 250 nm 2 ml 395 cd19m50 50 nm 2 ml 495
51 cd 43 - non b cd43m250 250 nm 2 ml 395 cd43m50 50 nm 2 ml 495
52 cd 45 ± Leukocytes cd45m250 250 nm 2 ml 395 cd45m50 50 nm 2 ml 495
53 cd 45R ± Leukocytes cd45rm250 250 nm 2 ml 395 cd45rm50 50 nm 2 ml 495
54 cd 90 ± T cells cd90m250 250 nm 2 ml 395 cd90m50 50 nm 2 ml 495
SECONDARY ANTIBODIES
55 goat anti Mouse IgG [H + L chains] gam250hl 250 nm 1 ml $400 gam50hl 50 nm 1 ml 800
56 Rat anti Mouse IgG 1 ram250 1 250 nm 2 ml 300 ram50 1 50 nm 2 ml 350
57 Rat anti Mouse IgG 2a ram250 2a 250 nm 2 ml 300 ram50 2a 50 nm 2 ml 350
58 Rat anti Mouse IgG 2b ram2502b 250 nm 2 ml 300 ram502b 50 nm 2 ml 350
59 Rat anti Mouse IgM ram250m 250 nm 2 ml 300 ram50m 50 nm 2 ml 350
60 Goat anti Rat IgG [H + L chains] gar250hl 250 nm 2 ml 300 gar50hl 50 nm 2 ml 350
60a Goat anti Mouse IgG Saporin Conjugated gams250 250 nm 2 ml 625 gams50 50 nm 2 ml 725
Particle Size/ List Particle Size List
ID COMMON CAPTURE/BASE PARTICLES Catalog # Volume Price Catalog # / Volume Price
61 Streptavidin sab250 250 nm 2 ml 350 sab50 50 nm 2 ml 400
62 Protein A pab250 250 nm 2 ml 325 pab50 50 nm 2 ml 375
63 Activated Particle Protein Coat [Amine groups] apb250 250 nm 1 ml 280 apb50 50 nm 1 ml 350
64 Protein Coated Magnetite pcm250 250 nm 1 ml 275 pcm50 50 nm 1 ml 350
65 Silica Coated Magnetite si250 250 nm 1 ml 300 si50 50 nm 2 ml 375
66 Carboxyl Function Protein Coat cb250 250 nm 1 ml 300 cb50 50 nm 1 ml 375
68 Sulffhydryl Function Protein Coat sf-250 250 nm 1 ml 350 sf-50 50 nm 2 ml 375
69 Avidin av250 250 nm 2 ml 300 av50 50 nm 2 ml 375
ANTIBODIES TO APOPTOSIS PROTEINS
70 Human Bcl-2 [B cell Lymphoma 2] bcl2-250 250 nm 2 ml 450 bcl2-50 50 nm 2 ml 550
71 Human Fas [CD95/APO-1] fas250 250 nm 2 ml 450 fas50 50 nm 2 ml 550
72 Human Fas Ligand T and NK Cells fasl250 250 nm 2 ml 500 fasl50 50 nm 2 ml 600
73 Mouse Fas [CD 95] fasm250 250 nm 2 ml 500 fasm50 50 nm 2 ml 600
74 Mouse Fas Ligand [CD 95 Ligand] fasml250 250 nm 2 ml 475 fasml50 50 nm 2 ml 600
75 Human p53 p53250 250 nm 2 ml 475 p5350 50 nm 2 ml 600
LABELED PARTICLES & ANTIBODIES
82 5 Bromo-2-Deoxyuridine [BrdU]. brd250 250 nm 2 ml 300 brd50 50 nm 2 ml 375
84 Green Fluorescent Protein gfp250 250 nm 1ml 350
85 Anti Alkaline Phosphatase aalk250 250 nm 2 ml 400 aalk50 50 nm 2 ml 500
86 Anti Brdu abrd250 250 nm 2 ml 300 abrd50 50 nm 2 ml 350
87 Anti FITC afit250 250 nm 2 ml 300 afit50 50 nm 2 ml 350
88 Anti Horseradish Peroxidase ahrp250 250 nm 2 ml 300 ahrp50 50 nm 2 ml 350
89 Anti Biotin abi250 250 nm 2 ml 275 abi50 50 nm 2 ml 350
90 Anti AFP [for the detection of GFP's] afp250 250 nm 2 ml 350 afp50 50 nm 2 ml 425
91 Blue Fluorescent Protein bfp250 250 nm 1 ml 350

Particle Size/

List Particle Size List
ID FUSION PROTEIN TAGS Catalog # Volume Price Catalog # / Volume Price
92 Anti c MYC [Human] myc250 250 nm 2 ml 325 myc50 50 nm 2 ml 395
93 Polyclonal Rabbit anti DNP dnp250 250 nm 2 ml 350 dnp50 50 nm 2 ml 400
94 Anti M1 flgm1250 250 nm 2 ml 375 flgm1-50 50 nm 2 ml 475
95 Anti M2 flgm2-250 250 nm 2 ml 375 flgm2-50 50 nm 2 ml 475
96 Anti M5 flgm5-250 250 nm 2 ml 375 flgm5-50 50 nm 2 ml 475
97 N-Terminal FLAG -BAP protein flgn250 250 nm 2 ml 350 flgn50 50 nm 2 ml 450
98 C-Terminal FLAG BAP protein flgb250 250 nm 2 ml 350 flgn50 50 nm 2 ml 450
99 Monoclonal mouse anti GST gst250 250 nm 2 ml 325 gst50 50 nm 2 ml 400
100 Polyclonal Rabbit anti HA ha250 250 nm 2 ml 350 ha50 50 nm 2 ml 450
101 5-Rox [Rhodamine] rox250 250 nm 2 ml 325 rox50 50 nm 2 ml 375
MOLECULAR BIOLOGY
102 Oligo dT oli250 250 nm 2 ml 350
61 Streptavidin sab250 250 nm 2 ml 350 sab50 50 nm 2 ml 400
121 DEAE DEAE250 250 nm 2 ml 395 deae50 50nm 2 ml 450
SEPARATORS, REAGENTS, & SUPPLIES
Description Price Use
l
.
Msp Small volume 250 nm separator 125 250 nm

Inquire about custom separators

MICROBIOLOGY PRODUCTS
103 Anti E. Coli 0157 Monoclonal 0157-200 250 nm 2 ml 375 O157-50 50 nm 2 ml $475
104 Prion Protein [PrP] monoclonal ,neurons,astro PrP250 250 nm 2 ml 375
105 Campylobacter jejuni cam250 250 nm 2 ml 375
106 H.pylori [ all antigens] hpy250 250 nm 2 ml 375
107 Listeria Monocytogenes [all antigens] lis250 250 nm 2 ml 375
108 Salmonella sp. [group] sal250 250 nm 2 ml 375
109 Anti E. coli 0157 Polyclonal O157-200P 200 nm 2 ml 375 O157-50P 50 nm 2 ml $475
Orders are taken by phone, fax, email, or mail. Please include your purchase order number. Prices are F.O.B. Belgium, and subject
to change without notice. Shipping and handling fees will be prepaid and reflected on the invoice. Orders are shipped by TNT Express