How to apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are excited by both lazers, and get data from computer?
GFP is the easiest excitation since it is much less toxic than PE excitation that might kill your cells.
This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)