Flow Cytometry is a widely used technique for identifying cell populations as well as measuring cell surface and intracellular molecules. Cells flow single file through a flow cytometer and multiple parameters can be collected and measured at a high-speed rate. This allows a large number of cells to be analyzed and sorted in a short amount of time. Flow Cytometry is used for research applications such as immunophenotyping, DNA studies, cell cycle analysis, and fluorescence-activated cell sorting (FACS).
The following flow cytometry staining protocols have been developed and optimized by R&D Systems Flow Cytometry Laboratory. These protocols are designed for intracellular or cell surface staining of proteins. It is recommended that experimental conditions, such as antibody concentration, incubation time, and temperature, be optimized for each flow cytometry experiment.
- Flow Cytometry Protocol for Cell Surface Markers
- Intracellular Staining Flow Cytometry Protocol using Detergents to Permeabilize the Cells
- Intracellular Staining Flow Cytometry Protocol Using Alcohol to Permeabilize the Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol