Product Description
anti- Flag tag antibody is available at Gentaur for Next week Delivery.
Purification: Protein A+G purification
Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is a rabbit polyclonal antibody raised against 3xFlag(3xDYKDDDDKT) sequence and recognizes the(3x)DYKDDDDK peptide and detects FLAG-tagged proteins..
Immunogen: Flag tag
Synonyms:
Reactivity: Recombinant protein
Tested Application: ELISA, WB, IP
Recommended dilution: WB: 1:1000-1:10000; IP: 1:10-1:100
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Image 3: IP Result of anti-Flag tag (IP: FNab03155, 3ug; Detection:2 FNab03155 1:2000) with Recombinant protein protein lysate 500ug.
Image 4: Flagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03155(Flag tag antibody) at dilution of 1:5000
Research Area: Metabolism