anti- P27; KIP1 antibody

Product Description

anti- P27; KIP1 antibody is available at Gentaur for Next week Delivery.

Purification: Immunogen affinity purified

Background: CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer..

Immunogen: cyclin-dependent kinase inhibitor 1B(p27, Kip1)

Synonyms: CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

Reactivity: Human, Mouse, Rat

Tested Application: ELISA, WB, IP, IHC, IF

Recommended dilution: WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500

Image 1: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06068(P27; KIP1 Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Image 2: Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using FNab06068( P27; KIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

Image 3: IP Result of anti-P27; KIP1 (IP:FNab06068, 4ug; Detection:FNab06068 1:300) with NIH/3T3 cells lysate 1600ug.

Image 4: NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab06068(P27; KIP1 Antibody) at dilution of 1:600

Gene ID: 1027

Research Area: Metabolism

Uniprot ID: P46527