anti- PCNA antibody

Product Description

anti- PCNA antibody is available at Gentaur for Next week Delivery.

Purification: Protein A+G purification

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion..

Immunogen: proliferating cell nuclear antigen

Synonyms: Proliferating cell nuclear antigen, Cyclin

Reactivity: Human, Mouse, Rat,rabbit

Tested Application: ELISA, WB, IHC, FC, IP

Recommended dilution: WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500

Image 1: Immunohistochemistry of paraffin-embedded human breast cancer using FNab06217(PCNA antibody) at dilution of 1:800

Image 2: Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using FNab06217 (PCNA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Image 3:

Image 4: HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06217(PCNA Antibody) at dilution of 1:10000

Gene ID: 5111

Research Area: Metabolism

Uniprot ID: P12004