Animal free protein surfaces for cell culture

Many mammalian and stem cell lines are heavily influenced by the surface upon which they grow. Even subtle changes can have dramatic effects - that’s why scientists need well characterised, animal-free growth surfaces like Orla’s.
Scientists using human and non-human stem cells, chondrocytes, neural cells, hepatocytes, and many other cell lines all use Orla products. They have used laminin,fibronectin, collagen and other motifs to show that:

• cell attachment, differentiation and fate can all be significantly affected and that short peptide motifs can be more powerful stimulants than corresponding whole proteins
• that motifs within a single protein can be synergistic or antagonistic in effect
• that the effect of using multiple motifs is sometimes predictable – but not always. This shows how complex life can be – but also why scientists need systems enabling them to study motif effects quickly, easily and cheaply.

The good news is that Orla has a library of animal-free proteins and peptide motifs allowing scientists to choose biological "membranes" of their choice. In our membranes:

• proteins are displayed as a highly orientated monolayer with chosen peptide motifs presented in natural protein loop conformations encountered in in-vivo conditions
• they are coated on a range of supplied ready to use in packs of 6, 12, 24, 48 or 96 items

Better still, you can decide on protein/motif concentration (this is controlled using "filler" molecules for dilution and to fill any remaining membrane gaps) and:

• You can choose mixtures and ratios of proteins and motifs; all you need to do is make the choice, and we will do the supply
• We can even pattern 2D surfaces: our fillers can be hydrophobic, hydrophilic or functionalised for patterning or printing surfaces e.g. the use of PEG to inhibit cell adhesion.

In other words, our surfaces are customised to your requirements, allowing you to do the experiments or assays of your choice. And … if our library doesn’t contain the proteins or motifs you require – simply provide us with your own motif cDNA. Our research team will test its incorporation into Orla’s membrane products.

To see our list of 2D and 3D surfaces and our library of peptide motifs