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    Macs Beads

    Posted by Lieven Gevaert, Gentaur BV on 14th May 2021

    Magnetic beads for cell separation

    Custom camel or camelid antibodies, nanobodies labeling with small beads for positive selection of 50 nm.

    All kind of tags can be added to you camle antibody. FLAG, GFP, HA.

    The beads and the fc will be integrated in your sell and expulsed by exocytosis.

    Except for CD11 monocytes that will be activated by the positive selection.

    CD11 cannot be selected by positive magnetic selection to be put back in culture.

    T cells, NK cell, B cells can be selected by the very small 50 nm beads. These beads are so small that since the first use of positive selection by Stephen Miltenyi in 1996 it would have been known if they prevent reculturing lymphocytes.

    However the cell state could be slightly changed by positive magnetic selection, like for monocytes CD11.

    Thats why we use 250 nm beads for negative selection. The 250 nm beads are much more preformant in taking out cells. As sales representative for Miltenyi we expetiences a lot of times in the laboratory of Professor Goldman in ULB Erasme that CD34 positive selection for reculture and reinjection in lymphocyte depleted patients was a perfect solution. This was before the use of the Clinimacs. Especially in 1998 in Liege Sart Tilman this was a much used protocol and we can now tell that these labs where the precursers of CD34 selection for in vivo therapeutic use.

    Lieven Gevaert, Bio-ir. Gent 1996, Scientific Director Gentaur BV, Brussels

    Vaslilyi Abaev, Ms. Biotechnology, Sofia 2009, Scientific custom conjugation Manager Gentaur LTd. London

    Camel beads are easlily biotinilated and thant couple to streptavidin magnetic beads

    ID HUMAN CELL SURFACE MOLECULES Catalog # Size/Volume

    		Price
    Euro    		 Catalog # Size/ Volume Price

     Euro

    1 cd 2 ± T Cells cd2 250 250 nm 2 ml 350 cd2 50 50 nm 2 ml 450
    2 cd 3 ± T Cells cd3 250 250 nm 2 ml 350 cd3 50 50 nm 2 ml 450
    3 cd 4 ± T Helper Cells cd4 250 250 nm 2 ml 350 cd4 50 50 nm 2 ml 450
    4 cd 5 ± Thymocytes cd5 250 250 nm 2 ml 375 cd5 50 50 nm 2 ml 475
    5 cd 6 ± Mature T Cells cd6 250 250 nm 2 ml 375 cd6 50 50 nm 2 ml 475
    6 cd 7 Thymocytes,T/pre B,NK cd7 250 250 nm 2 ml 375 cd7 50 50 nm 2 ml 475
    7 cd 8 ± T Cells cd8 250 250 nm 2 ml 350 cd8 50 50 nm 2 ml 450
    8 cd 9 platelets, pre B,mono,endothelia,epithilia cd9 250 250 nm 2 ml 375 cd9 50 50 nm 2 ml 475
    9 cd 10 ± Granulocytes cd10 250 250 nm 2 ml 375 cd10 50 50 nm 2 ml 475
    10 cd 11a/LFA 1 Leukocytes cd11a 250 250 nm 2 ml 375 cd11a 50 50 nm 2 ml 475
    11 cd 11b ± mono -Granulocytes cd11b 250 250 nm 2 ml 385 cd11b 50 50 nm 2 ml 500
    11c cd 11c NK Cells, Monocytes cd11c 250 250 nm 2 ml 375 cd11c 50 50 nm 2 ml 475
    12 cd 13 ± gran, mono,mast and GM progenitors cd13 250 250 nm 2 ml 375 cd13 50 50 nm 2 ml 475
    13 cd 14 ± mono cd14 250 250 nm 2 ml 385 cd14 50 50 nm 2 ml 500
    14 cd 15 ± Granulocytes cd15 250 250 nm 2 ml 410 cd15 50 50 nm 2 ml 500
    15 cd 16 ± NK cells cd16 250 250 nm 2 ml 375 cd16 50 50 nm 2 ml 475
    16 cd 18 ± monocytes, lymphocytes cd18 250 250 nm 2 ml 375 cd18 50 50 nm 2 ml 475
    17 cd B4419 dendritic cells, B cells cd19 250 250 nm 2 ml 395 cd19 50 50 nm 2 ml 475
    18 cd 20 pre,resting and activated B Cells cd20 250 250 nm 2 ml 375 cd20 50 50 nm 2 ml 475
    19 cd 21 ± B Cells dendritic and some epithelial cd21 250 250 nm 2 ml 375 cd21 50 50 nm 2 ml 475
    20 cd 22 ± B Cells cd22 250 250 nm 2 ml 395 cd22 50 50 nm 2 ml 475
    21 cd 23 ± mature B cells cd23 250 250 nm 2 ml 350 cd23 50 50 nm 2 ml 450
    22 cd 25 ± Activated T/B cells cd25 250 250 nm 2 ml 375 cd25 50 50 nm 2 ml 475
    23 cd 27 ± Thymocytes, Mature T lymphocytes cd27 250 250 nm 2 ml 375 cd27 50 50 nm 2 ml 475
    24 cd 31 plate, mono, gran,lympho,endothelial cd31 250 250 nm 2 ml 375 cd31 50 50 nm 2 ml 475
    25 cd 33 ± myeloid cells cd33 250 250 nm 2 ml 395 cd33 50 50 nm 2 ml 475
    26 cd 34 Progenitor cells cd34 250 250 nm 2 ml 395 cd34 50 50 nm 2 ml 475
    27 cd 45 all human leukocytes cd45 250 250 nm 2 ml 395 cd45 50 50 nm 2 ml 475
    28 cd 45 RO ± T Cells cd45ro250 250 nm 2 ml 395 cd45ro50 50 nm 2 ml 475
    29 cd 45 RA ± T, B, NK cells cd45ra250 250 nm 2 ml 395 cd45ra50 50 nm 2 ml 475
    30 HLA-DR ± antigen Presenting Cells hla250 250 nm 2 ml 385 hladr50 50 nm 2 ml 450
    31 cd 56 ± NK Cells cd56 250 250 nm 2 ml 395 cd56 50 50 nm 2 ml 450
    ID HUMAN CELL SURFACE MOLECULES Catalog # Volume Price Catalog # /Volume Price
    32 cd 62E Selectin endotheleum cd62e 250 250 nm 2 ml 395 cd62e 50 nm 2 ml 450
    33 cd 62 L Selectin Neutrophils, monocytes,T,B cd62l 250 250 nm 2 ml 395 cd62l50 50 nm 2 ml 475
    34 cd 62 P Selectin cd62p250 250 nm 2 ml 395 cd62p50 50 nm 2 ml 475
    35 cd 71 Transferrin Receptor cd71 250 250 nm 2 ml 450 cd71 50 50 nm 2 ml 475
    36 cd 90 [Thy-1] early cd34 and mature cd34 cd90 250 250 nm 2 ml 475 cd90 50 50 nm 2 ml 575
    37 cd 114 G-CSFR neutrophils,myeloid,endothel cd114 250 250 nm 2 ml 475 cd114 50 50 nm 2 ml 575
    38 cd 116 GM-CSFalpha, non blocking cd116 250 250 nm 2 ml 475 cd116 50 50 nm 2 ml 575
    39 cd 116 GM-CSFalpha,blocking cd116 250 250 nm 2 ml 475 cd116b50 50 nm 2 ml 575
    40 cd 121a IL-1R non blocking cd121a250 250 nm 2 ml 475 cd121a50 50 nm 2 ml 575
    41 cd 121a IL-1R blocking cd121ab250 250 nm 2 ml 475 cd121ab50 50 nm 2 ml 575
    42 cd 128 [IL-8RA] mono, gran cd128 250 250 nm 2 ml 475 cd12850 50 nm 2 ml 575
    43 cd 130 [gp 130] T,B cd130 250 250 nm 2 ml 375 cd130 50 50 nm 2 ml 450
    44 cd 152 [CTLA-4] cd152 250 250 nm 2 ml 475 cd152 50 nm 2 ml 575
    45 cd 154 [CD40L] Activated T cd154 250 250 nm 2 ml 475 cd154 50 50 nm 2 ml 575
    45a Anti Caveolin cav250 250 nm 2 ml 550 cav50 50 nm 2 ml 650
    45b Epithelial Specific Antigen esa250 250 nm 2 ml 400 esa50 50 nm 2 ml 475
    MOUSE CELL SURFACE MOLECULES
    46 cd 4 ± T cells cd4m250 250 nm 2 ml 395 cd4m50 50 nm 2 ml 495
    47 cd 5 ± T,B cells cd5m250 250 nm 2 ml 395 cd5m50 50 nm 2 ml 495
    48 cd 8a T cells cd8am250 250 nm 2 ml 395 cd8am50 50 nm 2 ml 495
    49 cd 11b ± Macrophages/Monocytes cd11bm250 250 nm 2 ml 395 cd11bm50 50 nm 2 ml 495
    50 cd 19 ± B cells cd19m250 250 nm 2 ml 395 cd19m50 50 nm 2 ml 495
    51 cd 43 - non b cd43m250 250 nm 2 ml 395 cd43m50 50 nm 2 ml 495
    52 cd 45 ± Leukocytes cd45m250 250 nm 2 ml 395 cd45m50 50 nm 2 ml 495
    53 cd 45R ± Leukocytes cd45rm250 250 nm 2 ml 395 cd45rm50 50 nm 2 ml 495
    54 cd 90 ± T cells cd90m250 250 nm 2 ml 395 cd90m50 50 nm 2 ml 495
    SECONDARY ANTIBODIES
    55 goat anti Mouse IgG [H + L chains] gam250hl 250 nm 1 ml $400 gam50hl 50 nm 1 ml 800
    56 Rat anti Mouse IgG 1 ram250 1 250 nm 2 ml 300 ram50 1 50 nm 2 ml 350
    57 Rat anti Mouse IgG 2a ram250 2a 250 nm 2 ml 300 ram50 2a 50 nm 2 ml 350
    58 Rat anti Mouse IgG 2b ram2502b 250 nm 2 ml 300 ram502b 50 nm 2 ml 350
    59 Rat anti Mouse IgM ram250m 250 nm 2 ml 300 ram50m 50 nm 2 ml 350
    60 Goat anti Rat IgG [H + L chains] gar250hl 250 nm 2 ml 300 gar50hl 50 nm 2 ml 350
    60a Goat anti Mouse IgG Saporin Conjugated gams250 250 nm 2 ml 625 gams50 50 nm 2 ml 725
    Particle Size/ List Particle Size List
    ID COMMON CAPTURE/BASE PARTICLES Catalog # Volume Price Catalog # / Volume Price
    61 Streptavidin sab250 250 nm 2 ml 350 sab50 50 nm 2 ml 400
    62 Protein A pab250 250 nm 2 ml 325 pab50 50 nm 2 ml 375
    63 Activated Particle Protein Coat [Amine groups] apb250 250 nm 1 ml 280 apb50 50 nm 1 ml 350
    64 Protein Coated Magnetite pcm250 250 nm 1 ml 275 pcm50 50 nm 1 ml 350
    65 Silica Coated Magnetite si250 250 nm 1 ml 300 si50 50 nm 2 ml 375
    66 Carboxyl Function Protein Coat cb250 250 nm 1 ml 300 cb50 50 nm 1 ml 375
    68 Sulffhydryl Function Protein Coat sf-250 250 nm 1 ml 350 sf-50 50 nm 2 ml 375
    69 Avidin av250 250 nm 2 ml 300 av50 50 nm 2 ml 375
    ANTIBODIES TO APOPTOSIS PROTEINS
    70 Human Bcl-2 [B cell Lymphoma 2] bcl2-250 250 nm 2 ml 450 bcl2-50 50 nm 2 ml 550
    71 Human Fas [CD95/APO-1] fas250 250 nm 2 ml 450 fas50 50 nm 2 ml 550
    72 Human Fas Ligand T and NK Cells fasl250 250 nm 2 ml 500 fasl50 50 nm 2 ml 600
    73 Mouse Fas [CD 95] fasm250 250 nm 2 ml 500 fasm50 50 nm 2 ml 600
    74 Mouse Fas Ligand [CD 95 Ligand] fasml250 250 nm 2 ml 475 fasml50 50 nm 2 ml 600
    75 Human p53 p53250 250 nm 2 ml 475 p5350 50 nm 2 ml 600
    LABELED PARTICLES & ANTIBODIES
    82 5 Bromo-2-Deoxyuridine [BrdU]. brd250 250 nm 2 ml 300 brd50 50 nm 2 ml 375
    84 Green Fluorescent Protein gfp250 250 nm 1ml 350
    85 Anti Alkaline Phosphatase aalk250 250 nm 2 ml 400 aalk50 50 nm 2 ml 500
    86 Anti Brdu abrd250 250 nm 2 ml 300 abrd50 50 nm 2 ml 350
    87 Anti FITC afit250 250 nm 2 ml 300 afit50 50 nm 2 ml 350
    88 Anti Horseradish Peroxidase ahrp250 250 nm 2 ml 300 ahrp50 50 nm 2 ml 350
    89 Anti Biotin abi250 250 nm 2 ml 275 abi50 50 nm 2 ml 350
    90 Anti AFP [for the detection of GFP's] afp250 250 nm 2 ml 350 afp50 50 nm 2 ml 425
    91 Blue Fluorescent Protein bfp250 250 nm 1 ml 350

    Particle Size/

    		 List Particle Size List
    ID FUSION PROTEIN TAGS Catalog # Volume Price Catalog # / Volume Price
    92 Anti c MYC [Human] myc250 250 nm 2 ml 325 myc50 50 nm 2 ml 395
    93 Polyclonal Rabbit anti DNP dnp250 250 nm 2 ml 350 dnp50 50 nm 2 ml 400
    94 Anti M1 flgm1250 250 nm 2 ml 375 flgm1-50 50 nm 2 ml 475
    95 Anti M2 flgm2-250 250 nm 2 ml 375 flgm2-50 50 nm 2 ml 475
    96 Anti M5 flgm5-250 250 nm 2 ml 375 flgm5-50 50 nm 2 ml 475
    97 N-Terminal FLAG -BAP protein flgn250 250 nm 2 ml 350 flgn50 50 nm 2 ml 450
    98 C-Terminal FLAG BAP protein flgb250 250 nm 2 ml 350 flgn50 50 nm 2 ml 450
    99 Monoclonal mouse anti GST gst250 250 nm 2 ml 325 gst50 50 nm 2 ml 400
    100 Polyclonal Rabbit anti HA ha250 250 nm 2 ml 350 ha50 50 nm 2 ml 450
    101 5-Rox [Rhodamine] rox250 250 nm 2 ml 325 rox50 50 nm 2 ml 375
    MOLECULAR BIOLOGY
    102 Oligo dT oli250 250 nm 2 ml 350
    61 Streptavidin sab250 250 nm 2 ml 350 sab50 50 nm 2 ml 400
    121 DEAE DEAE250 250 nm 2 ml 395 deae50 50nm 2 ml 450
    SEPARATORS, REAGENTS, & SUPPLIES
    Description Price Use
    l
    .
    Msp Small volume 250 nm separator 125 250 nm

    Inquire about custom separators
    MICROBIOLOGY PRODUCTS
    103 Anti E. Coli 0157 Monoclonal 0157-200 250 nm 2 ml 375 O157-50 50 nm 2 ml $475
    104 Prion Protein [PrP] monoclonal ,neurons,astro PrP250 250 nm 2 ml 375
    105 Campylobacter jejuni cam250 250 nm 2 ml 375
    106 H.pylori [ all antigens] hpy250 250 nm 2 ml 375
    107 Listeria Monocytogenes [all antigens] lis250 250 nm 2 ml 375
    108 Salmonella sp. [group] sal250 250 nm 2 ml 375
    109 Anti E. coli 0157 Polyclonal O157-200P 200 nm 2 ml 375 O157-50P 50 nm 2 ml $475
    Orders are taken by phone, fax, email, or mail. Please include your purchase order number. Prices are F.O.B. Belgium, and subject
    to change without notice. Shipping and handling fees will be prepaid and reflected on the invoice. Orders are shipped by TNT Express