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Product Description
Human TNF alpha ELKSA Kit is available at Gentaur for next week delivery.
Description: Sandwich ELKSA for quantitative detection of Human TNF alpha concentrations.
Appication: SE
Alternative Names: TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Reactivity: Human
Gene Symbol: TNF
Gene ID: 7124
Uniprot ID: P01375
Detection Method: Colorimetric
Detection Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: 7 pg/ml
Sample Types: Cell Culture Supernates, Serum, Plasma, Tissue Homogenates, Urine
Storage/Stability: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
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TNF - CACO2 cells
We used this kit to detect TNF-a in Caco2 cell lines. The assay is easy to use and the protocol provided is detailed and well explicated. As you can see from the graph we got a nice standard curve and result (low error bars).
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efficient and accurate
I used this kit to detect TNF-a in stimulated human CD14+ monocytes. The assay was extremely efficient. There is no overnight coating step, and detection/capture antibodies are added as a cocktail at the same time as the samples. The assay was quite accurate, detecting even minimal amounts of TNF-a. I would use this assay again for its efficiency, requiring very little time at the bench.
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works perfect
Works as expected.
