Product Description
Fluorescent Particles Ocean Blue 1% w/v 2.5-4.5 um is available at Gentaur for Next week Delivery.
At GENTAUR a wide variety of Fluorescent Particles is offered:
Fluorescent Particles are excellent tools for bioimaging and biosensing thanks to their stability and uniformal fluorescence. Fluorescent particles with functional groups for covalent binding of proteins, peptides and antibodies and particles covered with functuonal groups such as Amino groups, Carboxyl groups, Dimethylamino groups are available.:
• We offer a wide gam of particles with large range in terms of size, fluorophores, intensity of the fluorescence and various surface functional groups:
• The SPHERO fluorescent microparticles are produced by staining polystyrene particles with a fluorophore solution or by polymerizing a fluorophore in styrene in the presence of polystyrene core particles.:
• The fluorophores of our SPHERO fluorescent particles are NOT soluble in water which gives them a great stability.
SPHEROTM Flow Cytometry Multiplex Bead Assay Particles
SPHEROTM Flow Cytometry Multiplex Bead Assay Particles are designed for the development of flow cytometer
multiplex assays. These kits are used by companies or laboratories which develop assays for allergy testing, autoimmune
diseases, cardiac markers, cytokine detection, endocrine markers, infectious disease markers, isotyping, genotyping,
kinase and phosphorylated protein activity, metabolic markers, and tissue typing. For example, S. Chew, et al. "Stability
Screening of Arrays of Major Histocompatibility Complexes on Combinatorially Encoded Flow Cytometry Beads ." J.
Biol. Chem. 2011 286: 28466-28475. These kits are available with a variety of functionality, fluorophores and coatings.
SPHEROTM Blue Fluorescent Particle Array Kits (PAK)
• Designed to simplify flow cytometer multiplex assay development
• Consists of fluorescent particles of different intensities in PE-Cy5 channel and minimal
fluorescence in FITC and PE channels with 488 nm excitation
• Used with FITC and/or PE for detection
• Several sizes can be used independently or mixed together (3.5, 4.0 and 5.1 & 7.7
micron sizes available).
• Avaliable as Functionalized, Streptavidin, Biotin and antibody coated.
Blue PAK (Particle Array Kits) for multiplex flow assay are now available from Spherotech. The Blue PAK particles
are fluorescent in PE-Cy5, APC, and APC-Cy7 channel with minimal fluorescent in FITC and PE channels with 488 or
635 nm excitation. This allows that either FITC and/or PE tracers can be used for detection. These kits are available
with different functionalities and coatings. Carboxyl, amino and plain polystyrene functionalized particles are available
for covalent attachment or passive adsorption of ligands.
The Blue PAK and Carboxyl Blue PAK are available in 7.66 μm (9 peaks), 5.1 μm (10 peaks), 4.0 μm (8 peaks) and 3.6
μm (7 peaks) sizes. Blue PAK particles are also available coated with Streptavidin and Goat anti-Mouse IgG (H&L).
Most state-of-the-art single laser flow cytometers can resolve the 3.6 μm, 4.0 μm and 5.1μm Blue PAKs
easily in FSC/SSC channels. Ideally, one can mix them together and use both FITC and PE to run 50 assays in
the same tubes with most single laser flow cytometers.