The reagents will cause direct agglutination (clumping) of test red cells that carry the corresponding ABO antigen.
No agglutination generally indicates absence of the corresponding ABO antigen. Lorne Monoclonal IgM ABO blood grouping reagents contain murine monoclonal antibodies diluted in a phosphate buffer containing sodium chloride, EDTA and bovine albumin. Each reagent is supplied at optimal dilution for use with slide, tube, gel card & microplate techniques.