null
Toggle menu
020 3393 8531

    • We are part of the Gentaur group

    Shop by Category

  • 2019-nCoV Antibody
  • 2019-nCoV Protein
  • AccuCount
  • Allergen
  • Antibody
  • Antigen
  • Assay Developers
  • Assay Kit
  • Blocking Peptides
  • Cells
  • Clinical Laboratories
  • COVID-19
  • DNA
  • Elisa Kit
  • Elisa Kits
  • Extraction
  • Flow Cytometry
  • Gentaur Antibodies
  • Humanazied Antibody
  • Humanized monoclonal antibody
  • IBT Bioservices
  • ICL Antibodies
  • ICL ELISA
  • ICL Isotype Control
  • ICL Loading Control
  • ICL Protein Standard
  • Immuno-Histo Chemistry
  • Medium & Cell Culture
  • ORLA Gentaur
  • Oxidation
  • PCR Kit
  • Rabbit polyclonal antibody
  • SAH Antibodies
  • SAM Antibodies
  • SD Bioline
  • Serum
  • Stem Cell Marker
  • Virus
  • Antibodies
  • Assay
  • Cell
  • Coronavirus
  • ExoQuick
  • Fluorescence
  • Gel
  • Lab
  • Magnetic Beads
  • Molecular Biology
  • NatTrol
  • OneqPCR RealTime
  • Oxiselect
  • PAB Polyconals
  • Panbio
  • Plasmid
  • qPCR
  • QuantiChrom
  • Rainbow
  • Reagent
  • Recombinant
  • Solution
  • SPHERO
  • Spoligotyping
  • Stain
  • Monoclonal
  • Protein
  • RNA Isolation
  • Dounce homogenizer
  • Antigen ELISA
  • Assay Tests
  • Branched DNA Assay
  • Recombinant Proteins

    • Shop by Brand

  • Nova Tech
  • Biomatik Proteins
  • Fine Test
  • MBS
  • Life Science M
  • Mybiosource
  • Gentaur
  • Fine Test Ltd
  • Nova Life
  • Bioscience
  • Zeptometrix
  • Life Science Tech
  • Fine Biotech
  • Nova LifeTech
  • Gentaur Ltd.
  • Science Market
  • Immunology Consultatnt Laboratory
  • Wuhan Fine Biotech
  • Biovision
  • Genprice
  • Sphero Fluorescent
  • Bioworld
  • Gentaur Molecular
  • Genprice Inc.
  • 101Bio
  • Sphero Beads
  • IBT
  • Sphero Magnetic
  • Intact Genomics
  • Sphero Rainbow
  • Abbott
  • Stress Marq Biosciences
  • Jaica
  • ALPHAGEN Extractions
  • Arthus Bio
  • Eurofins
  • Sphero Blue
  • Chondrex
  • Sphero Calibration
  • Vazyme
  • Smart Science
  • Sphero Blank
  • Sphero Silica
  • Sphero Carboxyl
  • Abbott Panbio
  • AffiPLASMID
  • Beijing Wantai Biological
  • Invbio
  • Spherotech Beads
  • Vivadiag
  • View all Brands
  • Mouse Monoclonal Antibody to GAPDH

    • Mouse Monoclonal Antibody to GAPDH | Gentaur

    Gentaur

    €0.00
    (No reviews yet) Write a Review
    SKU:
    36-Y3322GAPDH-GEN
    Availability:
    24/48 H for Stock Items & 1 or 2 weeks for Production Items
    Weight:
    1 KGS
    Price:
    Contact info@gentaur.com for pricing

    Product Description

    Mouse Monoclonal Antibody to GAPDH

    Quality Level

    200

    biological source

    mouse

    conjugate

    unconjugated

    antibody form

    purified from hybridoma cell culture
    purified immunoglobulin

    antibody product type

    primary antibodies

    clone

    GAPDH-71.1, monoclonal

    form

    buffered aqueous solution

    mol wt

    antigen ~37 kDa

    species reactivity

    mouse, mink, rabbit, rat, human, hamster, canine, turkey, chicken, monkey, bovine

    should not react with

    prokaryotes

    General description

    Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH (glyceraldehyde-3-phosphate dehydrogenase). The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.

    Specificity

    Monoclonal Anti-GAPDH recognizes human, monkey, bovine, canine, rat, mouse, hamster, mink, rabbit, chicken, and turkey GAPDH. It does not cross-react with non-vertebrate and prokaryotic species.

    Immunogen

    Rabbit GAPDH.

    Application

    Monoclonal Anti-GAPDH antibody produced in mouse is suitable for western blotting using:
    • protein extracted from heart tissue of mice at a working dilution of 1:25,000
    • myelin and axogliasomal fractions from human CNS
    • nuclear and cytoplasmic fractions from TBP-13Q and TBP-105Q PC12 cells following recovery from heat shock
    • protein from bovine immortalized luteal endothelial cells
    • renal tubular epithelial cell extract
    • proteins from mouse embryonic fibroblasts
    • protein extract from ventricular myocardium tissues
    • A431 total cell extract at a working concentration of 0.025-0.05μg/mL
    It is also suitable for immunostaining using leiomyomas and leiomyosarcomas. The antibody can also be used for immunocytochemistry, indirect ELISA and microarray.

    Biochem/physiol Actions

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH.
     
    Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress.
     
    GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.

    Physical form

    Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

    Storage and Stability

    For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

    Disclaimer

    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    Product Videos

    Custom Field

    Price Contact info@gentaur.com for pricing

    Product Reviews

    Write a Review

    Recommended