Species Reactivity Human
Host/Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Conjugate FITCSee Additional Formats
Excitation/Emission Profile View spectra
Form Liquid
Purification purified
Storage buffer PBS with BSA
Contains 0.1% sodium azide
Storage conditions 4° C, store in dark
RRID AB_2536550
Target IgG
Antibody Form Whole Antibody
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence).
They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.