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  • GluR2 Antibody

    • GluR2 Antibody | Gentaur

    Gentaur

    €0.00
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    SKU:
    355-12758-2-GEN
    Availability:
    24/48 H for Stock Items & 1 or 2 weeks for Production Items
    Weight:
    1 KGS
    Price:
    Contact info@gentaur.com for pricing

    Product Description

    GluR2 Antibody

    Quality Level

    100

    biological source

    mouse

    antibody form

    purified immunoglobulin

    antibody product type

    primary antibodies

    clone

    6C4, monoclonal

    species reactivity

    monkey

    species reactivity (predicted by homology)

    rat, mouse, canine

    manufacturer/tradename

    Chemicon®

    technique(s)

    ELISA: suitable
    immunocytochemistry: suitable
    immunohistochemistry: suitable
    immunoprecipitation (IP): suitable
    radioimmunoassay: suitable
    western blot: suitable

    isotype

    IgG2a

    Specificity

    Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2.
     
    Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996).

    Application

    Immunocytochemistry:
    on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).

    ELISA/RIA:
    A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).

    Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).

    Immunoprecipitation: 2-4 µg/mL (Osten, 1998).

    Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.

    Optimal working dilutions and protocols must be determined by end user.
    Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB with more than 50 product citations.

    Quality

    Routinely evaluated by Western Blot on mouse brain lysates.

    Western Blot Analysis:
    1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.

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