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Subcloning
subcloning
Subcloning is a basic procedure in molecular biology for transfer of DNA inserts from one vector to another to gain functionality to study the sequence of interest. Essentially all subcloning reactions proceed the same way as illustrated in the figure below. You release and purify your insert from the parent vector, ligate this insert into a prepared destination vector, and transform this ligation reaction into competent bacterial cells. Then, you screen the transformed cells for the inserted DNA