Phalloidin staining protocol including buffers, reagents and a detailed procedure for staining with phalloidin dye conjugates.

Phalloidin is a highly selective bicyclic peptide that is used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of animals and plants.

Typically, it is used conjugated to a fluorescent dye, such as FITC, Rhodamine, TRITC or similar dyes, such as Alexa Fluor® 488 or iFluor 488.

Phalloidin can be used with sample types such as formaldehyde-fixed and permeabilized tissue sections, cell cultures and cell-free experiments. It can also be used in paraffin-embedded samples that have been de-paraffinized.