Product Description
Recombinant Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA), partial (Active) is available at Gentaur for Next week Delivery.
Gene Name: SIRPA
Alternative Names : Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; SHP Substrate 1; SHPS-1; Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs; Bit; CD172 Antigen-Like Family Member A; Inhibitory Feceptor SHPS-1; Macrophage Fusion Receptor; MyD-1 Antigen; Signal-Regulatory Protein Alpha-1; Sirp-Alpha-1; Signal-Regulatory Protein Alpha-2; Sirp-Alpha-2; Signal-Regulatory Protein Alpha-3; Sirp-Alpha-3; p84; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP
Expression Region : 31-370aa
AA Sequence : EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER
Sequence Info : Partial
Tag Info : C-terminal 6xHis-tagged
Theoretical MW : 38.31 kDa
Storage Buffer : Lyophilized from a 0.2 ?m filtered 20 mM PB, 150 mM NaCl, pH 7.2
Endotoxin Level : Less than 1.0 EU/µg as determined by LAL method.-
Biological Activity : The ED50 as determined by its ability to bind Human CD47 in functional ELISA is less than 0.5 ug/ml
Storage : Short term: -20°C; Long term: -80°C. Minimize freeze and thaw cycles.
Research Area : Cardiovascular
Restriction : For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Relevance : Signal Regulatory Protein ? (SIRP?) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRP? contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRP? can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRP? acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRP? shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRP? engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation
Function : Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Involvement in disease :
Subcellular location : Membrane, Single-pass type I membrane protein
Protein Families :
Tissue Specificity : Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Paythway : Osteoclastdifferentiation
Uniprot ID : P78324