Mouse Anti S-AdenosylHomocysteine (SAH) Clone 839-6 | MA00307

Product Description

Mouse Anti S-AdenosylHomocysteine (SAH) Clone 839-6 | MA00307 | Arthus Biosystems

Product name         

Mouse anti-SAH 7a/b

Catalog Number     

MA00307-50/100

Description              

Mouse monoclonal antibody against S-Adenosylhomocysteine [839-6]

Specificity                 

MA00307 shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: -1.3%, Adenosine: < 1%, Homocysteine: < 1%, L-Cysteine: < 1%, Glutathione: < 1%, L-Cystathionine: < 1%; Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%.

Immunogen              

S-Adenosylhomocysteine conjugated to BSA

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH7.4 (NaCI 150mM), Sodium azide 0.02%, BSA 10mg/m1 or PBS 10mM, pH7.4 (NaCI 150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/m1

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                    

839-6

Immunoglobin isotype      

IgG2a

Affinity                               

Ka = 7.38 x 109L/mol     ( 1.35 x 10-19M   )

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MA00307 in the following tested applications has been tested. The titer was measured using indirect assay with the concentration of the antibody at 1mg/ml. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.

Notes

cELISA: 1:30,000/36,000

FCM:  1:300

IHC:  1:300

Target

S-adenosylhomocysteine is a competitive inhibitor of S-adenosylmethionine-dependant methyl transferase reactions. Therefore, it plays a key role in the control of methylation via regulation of the intracellular concentration of S-adenosylhomocysteine.

Cellular localization  

Cytoplasm, nuclear

Figure 1: Titration curve for purified monoclonal antibody 839-6 at 1mg/ml.

Figure 2: Competitive ELISA using anti-S-Adenosymethionine monoclonal antibody [839-6] (MA00307)

The 0.2 pg/ml of SAH-BSA was coated into 96 wells. Serial dilution of SAH standard (SAHNa), S-Adenosylmethionine SAM: Sigma-Aldrich Cat# A2408), Homocysteine (H-Cys), L-Cysteine (L-Cys), Adenosine (Ade), Glutathione (GST), L- Cystathionine (L-CTT), Methythioadenosine (MTA), ADP (adenosine diphosphate), ATP (adenosine triphosphate) and  properly diluted   MA00307 were added. HRP conjugated Goat anti-Mouse IgG antibody was used to develop the color. 0D450 value was measured on each well.

Figure 3: Immunohistochemistry staining was performed using MA00307 with benign breast tissue adjacent to breast cancer. Brown areas indicated positive staining in nuclear and cytoplasmic areas. Strong HE staining may affect nuclear SAH positive signals (x400).