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Product Description
Sensitive Tissue Collagen assayis available at Gentaur for Next week Delivery.
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Total Collagen Assay Kit Can Be Used to Measure Collagen in Cell Media
This kit in the past has been used to measure total collagen in tissue, serum, and urine however, this kit as well as many other on the market are not tested for cell culture media. Production of Type 1 collagen is a common marker of cells under going differentiation due to an epithelial to mesenchymal (EMT) type process. Here we treated primary fetal membrane cells with oxidative stress inducer (cigarette smoke extract CSE) and measured the amount of total collagen they secreted in 48 hours. To our surprise we were able to detect low levels of collagen from cell media with in the kit range of 0.1ug/ul - 1ug/ul. To achieve this we used the protocol for urine samples, including adding 4 mg of activated charcoal to each sample to remove the color. However, we did have to individually test each sample to make sure it was neutral. Researchers running this assay on cell media in the future should beware that adding 10N NAOH before hydrolysis and then 10N of HCL after as stated in the book did not neutralize the cells media samples. Overall, this kit is able to detect low levels of collagen in cell media.
