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Product Description
anti- BrdU antibody is available at Gentaur for Next week Delivery.
Purification: Protein A+G purification
Background: Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine..
Immunogen: compound
Synonyms: 5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR
Reactivity: Human, Mouse, Rat
Tested Application: ELISA, IF, IHC
Recommended dilution: IHC: 1:200-1:1000; IF: 1:50-1:500
Image 1: Immunohistochemistry of paraffin-embedded mouse spleen tissue slide(with/without BrdU feeding) using FNab00952(BrDu Antibody) at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Image 2: Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using FNab00952 ( BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Research Area: Metabolism
