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Product Description
anti- GADD34 antibody is available at Gentaur for Next week Delivery.
Purification: Immunogen affinity purified
Background: GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4(ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase(PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and this antibody primarily recongize the 100 kDa band..
Immunogen: protein phosphatase 1, regulatory(inhibitor) subunit 15A
Synonyms: GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A
Reactivity: Human, Mouse, Rat
Tested Application: ELISA, WB, IHC, IF
Recommended dilution: WB: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Image 1: Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab03293(GADD34 antibody) at dilution of 1:100
Image 2: Immunofluorescent analysis of PC-3 cells using FNab03293( GADD34 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Image 3:
Image 4: K-562 cells were subjected to SDS PAGE followed by western blot with FNab03293(GADD34 antibody) at dilution of 1:600
Research Area: Metabolism
Uniprot ID: O75807
