anti- UBE2T/HSPC150 antibody

Product Description

anti- UBE2T/HSPC150 antibody is available at Gentaur for Next week Delivery.

Purification: Immunogen affinity purified

Background: The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways..

Immunogen: ubiquitin-conjugating enzyme E2T(putative)

Synonyms: HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T

Reactivity: Human, Mouse, Rat

Tested Application: ELISA, WB, IF, IP

Recommended dilution: WB: 1:200-1:2000; IP: 1:200-1:2000?IF?1:50-1:500

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Image 4: Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800

Gene ID:

Research Area: Metabolism

Uniprot ID: Q9NPD8