anti- USP28 antibody

Product Description

anti- USP28 antibody is available at Gentaur for Next week Delivery.

Purification: Immunogen affinity purified

Background: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome(APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7(FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7(FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) in a subset of colorectal cancers(CRC) cells(PubMed:24623306)..

Immunogen: ubiquitin specific peptidase 28

Synonyms: Deubiquitinating enzyme 28, KIAA1515, Ubiquitin thiolesterase 28, USP28

Reactivity: Human, Mouse, Rat

Tested Application: ELISA, WB, IHC, IP

Recommended dilution: WB: 1:500-1:5000; IP: 1?200-1?2000; IHC: 1?20-1?200

Image 1: Immunohistochemistry of paraffin-embedded human breast cancer using FNab09322(USP28 antibody) at dilution of 1:50

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Image 4: HeLa cells were subjected to SDS PAGE followed by western blot with FNab09322(USP28 antibody) at dilution of 1:1000

Gene ID: 57646

Research Area: Metabolism

Uniprot ID: Q96RU2