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Cell culture is one of the major techniques in the life sciences. It is the general term used for the removal of cells, tissues or organs from an animal or plant and their subsequent placement into an artificial environment conducive to their survival and/or proliferation. Basic environmental requirements for cells to grow optimally are: controlled temperature, a substrate for cell attachment, and appropriate growth medium and incubator that maintains correct pH and osmolality. The most important and crucial step in cell culture is selecting appropriate growth medium for the in vitro cultivation. A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms, cells, or small plants. Cell culture media generally comprise an appropriate source of energy and compounds which regulate the cell cycle. A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors. In addition to nutrients, the medium also helps maintain pH and osmolality.