Recombinant EBOV-Makona GPDTM (S2)

Product Description

Recombinant EBOV-Makona GPDTM (S2) is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: ELISA, WB

Description: Mature, recombinant, His-tagged Ebola virus Glycoprotein minus the transmembrane domain (EBOV rGPΔTM – Makona strain) is supplied as purified protein. EBOV rGPΔTM is produced in stably-transfected Drosophila Schneider 2 (S2) cells and is purified by FPLC.

Storage: 2-3 weeks at -20°C, -80°C long term

Size: 100 µg of protein supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 1.346 mg/mL.

Relevance: Recombinant glycoprotein provides a means as a control protein for immunoassays and a tool to enhance research.

Western Blot: Quality control testing demonstrates strong detection of GP under reduced conditions.

SDS-PAGE & Western Blot Detection

(A) SDS-PAGE and stain demonstrating 1 µg and 5 µg
(lanes 1, 2 respectively) of EBOV rGPΔTM His-tag protein
under denaturing and reducing conditions. MW denotes
Novex® Sharp prestained protein marker. (B) Western
blot detection of EBOV rGPΔTM at 50 ng, 100 ng and 500
ng (lanes 1-3) using rabbit anti-EBOV GPΔmuc pAb
(catalog# 0301-038) at 2.0 g/mL and anti-rabbit IgGHRP conjugate,                                                                      followed by TMB membrane substrate.

ELISA Data

Plate was coated with EBOV rGPΔTM starting at 800
ng/well, serially diluted 2-fold in DPBS. Washed plate
was detected using one dilution of a positive control
serum, followed with anti-IgG HRP conjugate and TMB
substrate. OD650 is reported. Background of EBOV
rGPΔTM coated plate positive control serum was 0.063
OD650.