Recombinant EBOV VP24

Product Description

Recombinant EBOV VP24 is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: ELISA, WB

Description: Recombinant, HIS-tagged, purified Ebola virus (EBOV) matrix protein (VP24) is
expressed in E. coli and the recombinant protein purified using FPLC.

Storage: -80°C. It is recommended to dispense single-use aliquots and store aliquots at -80°C to
avoid multiple freeze/thaw cycles.

Size: 100 µg of protein is supplied in Tris buffered saline, at a concentration of 2.279 mg/mL. The theoretical molecular weight of the protein is ~25 kDa.

Relevance: Recombinant EBOV VP24 matrix protein provides a means as a control protein for immunoassays and a tool to enhance Filovirus research.

SDS-PAGE: Quality control testing shows a prominent protein band above 20 kDa (VP24).

Western Blot: Assay-dependent dilution

ELISA: Assay-dependent dilution

SDS-PAGE and Western Blot

(A) SDS-PAGE and stain demonstrating 1 ug and 5
ug (lanes 1-2) of EBOV VP24 protein under
denaturing and reducing conditions. MW denotes
Novex® Sharp pre-stained protein markers. (B)
Anti-EBOV VP24 antibody (0301-047) at 100
ng/mL detected 100 ng of EBOV rVP24 (lane 1) by
Western Blot Analysis. Western Blots were
developed using an anti-rabbit IgG-HRP conjugate
and TMB Membrane substrate. MW denotes
Novex® Sharp pre-stained protein markers.

ELISA Data:

rVP24 protein was diluted to 3 µg/mL in PBS for
plate coating. Anti-EBOV VP40 antibody was
serially diluted semi-log from 10 µg/mL and
incubated on the coated plates. Washed plates
were detected with anti-rabbit IgG-HRP conjugate
and TMB substrate. OD650 is reported above.