Anti Dibromo-Tyrosine (DiBrY) monoclonal antibody

Product Description

Anti Dibromo-Tyrosine (DiBrY) monoclonal antibody is available at Gentaur for Next Week Delivery.

Description:  Neutrophils and eosinophils play an important role in the defence system against microbial infection. Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are known to catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr). These reactive intermediates may react with proteins, lipids and nucleotides, and it has been reported to form tyrosine halogenation such as dibromotyrosine (DiBrY). DiBrY is a Br-modified tyrosine at 3- and 5- position, which is one of the major oxidative products derived from neutrophil myeloperoxidase.

More Information:  Monoclonal antibody clone 3A5 have been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo). This antibody is specific for 3,5-Dibrominated tyrosine and other di-halogenated tyrosine at 3- and 5- position. Suitable for immunohistochemical analysys of oxidative stress.

Assay principle: 

Specifity:  This antibody can react with dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Cross reactivity to following analogues have been tested: dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly react with Bromotyrosine (BrY).

Measuring range: 

Form: Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG.

Antigen: 3,5-dibromotyrosine conjugated KLH(keyhole limpet hemocyanin).

Applications:  Immunohistochemistry, western blotting and ELISA.

Storage:  Less than -20°C

Expiry: 

Required but not provided: 

Remarq: