GRP78 (Bip) Protein

Product Description

GRP78 (Bip) Protein is available at Gentaur for Next week delivery.

Description:  Human Recombinant GRP78 (Bip) Full Length Protein

Alternative Name(s):  BIP Protein, Grp78 Protein, HSPA5 Protein, MIF2 Protein, immunoglobulin heavy chain binding Protein

Research Area(s):  Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Organelle Markers

Nature:  Recombinant

Accession Number: NM_005347

Gene ID:  3309

Swiss-Prot:  P11021 

Applications Species:  WB | SDS-PAGE | ATPase Activity Assay | Functional Assay | ELISA 

Biological Activity: 

Expression System: E. coli

Protein Length:  Full Length

Amino Acid Sequence: MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL

Purification:  Affinity Purified

Storage Buffer:  20mM Tris/HCl pH7.5, 50mM KCl, 5mM MgCl2, 1mM DTT

Concentration:  Lot/batch specific. See included datasheet.

Shipping Temperature: Blue Ice or 4ºC

Other relevant information:

Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis and gamma globulin as the protein concentration standard. 

Cellular Localization:  Endoplasmic Reticulum Lumen | Melanosome

Scientific Background:  GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

References: 1. Cho S., et al (2007) Mol Cell Biol. 27(1): 368-83. 2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555. 3. Luo S., et al (2006) 26(15): 5688-97. 4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949. 7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.

Field of Use:  Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

PubMed ID:  28464871|24853418|23858031|23448667|23049684

Published Application:  Western Blot Control|Protein Binding Assay|ELISPOT|ATPase activity assay|ATPase activity assay

Published Species Reactivity:  |Chemical compound|Mouse||