Recombinant Escherichia coli KHG/KDPG aldolase (eda)

Product Description

Recombinant Escherichia coli KHG/KDPG aldolase (eda) is available at Gentaur for Next week Delivery.

Gene Name: eda

Alternative Names : 2-keto-4-hydroxyglutarate aldolase;KHG-aldolase2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14);2-keto-3-deoxy-6-phosphogluconate aldolase;KDPG-aldolasePhospho-2-dehydro-3-deoxygluconate aldolasePhospho-2-keto-3-deoxygluconate aldolase

Expression Region : 1-213aa

AA Sequence : MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL

Sequence Info : Full Length

Tag Info : N-terminal 6xHis-tagged

Theoretical MW : 26.3 kDa

Storage Buffer : Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Endotoxin Level : Not tested-

Biological Activity : Not tested

Storage : Short term: -20°C; Long term: -80°C. Minimize freeze and thaw cycles.

Research Area : Others

Restriction : For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.

Relevance : Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.

Function : Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.

Involvement in disease :

Subcellular location : Cytoplasm

Protein Families : KHG/KDPG aldolase family

Tissue Specificity :

Paythway :

Uniprot ID : P0A955