Product Description
HRP Anti-SAM Monoclonal Antibody Clone 118-18 | MAH00203-50 | Arthus Biosystems
Product name
HRP-anti-SAM 3a
Catalog Number
MAH00203-50
Description
Horseradish peroxidase (HRP) conjugated anti-S-adenosylmethionine monoclonal antibody clone 118-18
Specificity
MAH00203 shows the following reactivities with related compounds: S-Adenosylmethionine: 100%, S-Adenosylhomocysteine: < 1% ,Adenosine: < 1%, L-Methionine: < 1%, Methythioadenosine (MTA): < 1%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%
Immunogen
S- Adenosylmethionine analog Aza-SAM conjugated to KLH.
Properties
Form
Liquid
Storage instructions
Store at 4°C, -20°C for long term storage
Storage buffer
PBS 10mM pH 7.4 (NaCI 150mM), Proclin 0.1%, Glycerol 50%, BSA 10mg/mi
Purity
>95% Purified from mouse ascites fluid by affinity chromatography
Clonality
Monoclonal
Clone number
118-18
Immunoglobin isotype
IgG2b
Research Areas
- Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
- One-carbon metabolism
- Signal Transduction
- Metabolism
- Pathways and Processes Cancers
- Arthritis
- Heart diseases
- Neurodegenerative diseases
- Atherosclerosis
- Liver diseases
- Kidney diseases
Applications
The use of MAH00203 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications. Refer to Cat # MA00203 for details.
Notes
cELISA: 1/2,000 - 1/10,000 (depending on the amount of coating)
Figure 1: The standard curve with HRP-anti-SAM 3 in direct competitive ELISA using 4-parameter logistic non-linear regression model. The 0.25 ug/m1 of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. Serial dilution of SAM standard (Cat # AST00201) was added along with 1:10,000 diluted HRP-anti-SAM 3 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.