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The flow cytometry protocols below provide detailed procedures for the treatment and staining of cells prior to using a flow cytometer.
Protocols are available for:
- Direct staining of cells applicable where the fluorophore is directly linked to the primary antibody
- Indirect staining of cells applicable when using unconjugated or biotin-conjugated monoclonal and polyclonal antibodies
- Intracellular staining methods for intracellular antigens and cytokines
- DNA staining for cell cycle analysis
- Cell preparation