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Flow Protocols

Flow Protocols

The flow cytometry protocols below provide detailed procedures for the treatment and staining of cells prior to using a flow cytometer.

Protocols are available for:

  • Direct staining of cells applicable where the fluorophore is directly linked to the primary antibody
  • Indirect staining of cells applicable when using unconjugated or biotin-conjugated monoclonal and polyclonal antibodies
  • Intracellular staining methods for intracellular antigens and cytokines
  • DNA staining for cell cycle analysis
  • Cell preparation

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