Product Description
Rabbit anti-EBOV GP pAb is available at Gentaur for Next Week Delivery.
Product Type: polyclonal antibody
Host: Rabbit
Clonality: polyclonal
Applications: ELISA, WB
Immunogen: Peptide sequence to Ebola Virus (EBOV) glycoprotein (GP).
Description: Affinity purified rabbit polyclonal antibody reactive to EBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant GP without the transmembrane region (rGPdTM) in Western blot and ELISA.
Supplied: 100 µg of antibody is supplied in PBS +0.02% sodium azide at a concentration of 1.09 mg/mL.
Purification: Antibody is affinity purified using immobilized immunogen.
Relevance: The antibody can be used for detection of EBOV GP.
Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution. Internal QC demonstrates good detection of GP when using antibody at 0.1 µg/mL.
Storage: 2-3 weeks +4oC, -20◦C long term Cross Reactivity: No cross reactivity to SUDV or MARV GP was observed in Western blotting.
Signals were slightly higher than background signals in ELISA against SUDV and MARV VLP only at the high concentrations of antibody.
Western Blot Data:
Western blots were detected under reduced
conditions with anti-EBOV GP antibody at 0.1
µg/mL and visualized using an anti-rabbit IgG-HRP
conjugate and TMB Membrane substrate. GP is
visualized in EBOV rGPdTM (lane 1) and in
Baculovirus-expressed EBOV VLP (lane 4). No
cross-reactivity with SUDV or MARV GP was
observed.
ELISA Data:
rGPdTM proteins and VLPs were diluted to 1
µg/mL and 10 µg/mL, respectively, in PBS for plate
coating. Anti-EBOV GP antibody was serially
diluted semi-log from 10 µg/mL and incubated on
the coated plates. Washed plates were detected
with anti-rabbit IgG-HRP conjugate and TMB
substrate. OD650 is reported above