Rabbit anti-EBOV L-Polymerase pAb

Product Description

Rabbit anti-EBOV L-Polymerase pAb is available at Gentaur for Next Week Delivery.

Product Type: polyclonal antibody

Host: Rabbit

Clonality: polyclonal

Applications: ELISA, WB

Immunogen: Peptide sequence specific to Zaire Ebola virus (ZEBOV) L-Polymerase protein.

Description: Affinity purified rabbit polyclonal antibody reactive to Zaire Ebola virus L-Polymerase.
The antibody detects L-Polymerase by Western blot in ZEBOV infected HeLa cells.

Supplied: 500 µg of affinity purified antibody is provided in PBS at a concentration of 0.93 mg/mL.
0.01% Sodium azide has been added.

Purification: Antibody is affinity purified using immobilized immunogen.

Relevance: The antibody can be used for detection of ZEBOV L-Polymerase

Storage: 2-3 weeks +4oC, -20◦C long term. Avoid multiple freeze thaws.

Specificity Testing:
ELISA: Not Tested.
WB: Western blot detection was performed to determine the cross-reactivity of the antibody to virus
like particles (VLPs) of Zaire and Sudan ebola virus (ZEBOV and SEBOV) and Marburg virus (MARV) expressing glycoprotein, nucleoprotein and VP40.
Reactivity to a ZEBOV (Mayinga) ebolavirus infected HeLa cells and uninfected HeLa cells were also
evaluated by Western Blot. Cell lysates were prepared in PBS and lysed with RIPA buffer plus protease inhibitors.

Western Blot Data

Western blot performed with uninfected HeLa cells (Lane 1) and HeLa cells infected with ZEBOV (Mayinga)
ebolavirus (Lane 2). Blots were detected using anti-L Polymerase at 1 µg/mL and visualized using an antirabbit HRP conjugate and chromogenic substrate (ZEBOV L polymerase is shown by arrow). Data kindly provided by Dr. Olena Shtanko in the lab of Dr. Robert Davey at the Texas Biomedical Research Institute.

Specificity: Antibody detects L-polymerase at the expected size of approximately 240 kDa in the HeLa
infected lysate (Lane 2), but not the control uninfected lysate (Lanes 1).

Cross Reactivity: Western Blot analysis shows that the antibody very weakly detects a band in 2 µg of MARV
VLPs between 30-40 kDa but not ZEBOV and SEBOV VLPs. Also, in addition to detection of the expected Lpolymerase, the anti-L antibody detects two bands in the uninfected HeLa lysate between 35-52 kDa. The target of this reactivity is unknown. Reactivity of the antibody to L polymerase of other filovirus species is
also unknown.
Intended for research use only, not for human, therapeutic, or diagnostic applications.