Rabbit anti-EBOV VP40 pAb

Product Description

Rabbit anti-EBOV VP40 pAb is available at Gentaur for Next Week Delivery.

Product Type: polyclonal antibody

Host: Rabbit

Clonality: polyclonal

Applications: ELISA, WB

Immunogen: Peptide sequence specific to Ebola Virus (EBOV) VP40 matrix protein.

Description: Affinity purified rabbit polyclonal antibody reactive to EBOV VP40. The antibody detects VP40 in viruslike particles (VLP) and recombinant VP40 (rVP40) in Western Blot and ELISA.

Supplied: 100 µg is supplied in PBS + 0.02% sodium azide
at a concentration of 0.789 mg/mL.

Raised in: Rabbits

Purification: Antibody is affinity purified using immobilized immunogen.

Relevance: The antibody can be used for detection of EBOV
VP40 matrix protein.

Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution.

Storage: 2-3 weeks +4oC, -20◦C long term. Avoid multiple
freeze thaws.

Cross Reactivity: Cross-reactivity was observed against Sudan Virus (SUDV) VP40 in VLP and recombinant protein in the ELISA. No cross reactivity was observed with Marburg Virus (MARV) rVP40.

Western Blot Data:

Anti-EBOV VP40 antibody at 10 ng/mL was able to detect
EBOV VP40 in EBOV VLPs and at least 100 ng of EBOV
rVP40 by Western Blot Analysis. Western Blots were
developed using an anti-rabbit IgG-HRP conjugate and TMB
Membrane substrate. No cross-reactivity with MARV VP40
was observed.

ELISA Data:

VLPs and rVP40 proteins were diluted to 10 µg/mL and 3
µg/mL, respectively, in PBS for plate coating. Anti-EBOV
VP40 antibody was serially diluted semi-log from 10 µg/mL
and incubated on the coated plates. Washed plates were
detected with anti-rabbit IgG-HRP conjugate and TMB
substrate. OD650 is reported above. The anti-EBOV VP40
antibody strongly reacted to EBOV VP40 in VLP and rVP40.
Cross-reactivity was observed against SUDV VP40 in VLP
and rVP40. Signals were observed against MARV VLP at the
highest concentrations of antibody tested