Recombinant EBOV GPDmuc (Sf9)

Product Description

Recombinant EBOV GPDmuc (Sf9) is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: ELISA, WB

Description: Recombinant, purified Ebola virus (EBOV) rGPΔmuc protein with HIS-Tag is produced in Sf9 insect cells using
baculovirus for expression and is purified by FPLC.

Storage: -80◦C

Size: 100 µg/vial is supplied PBS, pH7.4 (supplemented with glycerol, arginine and glutamic acid) at a concentration at a
concentration of 0.968 mg/mL. The theoretical molecular weight of the protein is 56 kDa.

Relevance: Recombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to
enhance research.

SDS-PAGE & Western Blot Detection

(Panel A) SDS-PAGE demonstrating 5 ug and 1 ug (lane 1, 2
respectively) of EBOV rGPΔmuc protein under denaturing and
reducing conditions. MW denotes Novex® Sharp pre-stained
protein standard.
(Panel B) Western blot detection of EBOV rGPΔmuc at 500 ng, 100
ng and 50 ng (lanes 1-3) under denaturing and reducing
conditions. EBOV rGPΔmuc was detected using an anti-EBOV
rGPΔmuc polyclonal antibody at 2.0 g/mL and anti-rabbit IgGHRP                                                                          conjugate, followed by TMB membrane substrate.

ELISA Data

Plate was coated with EBOV rGPΔmuc starting at 800 ng/well,
serially diluted in DPBS. The washed plate was detected using one
dilution of a positive control serum, followed with anti-IgG-HRP
conjugate and TMB microwell substrate. OD650 is reported.
Background of EBOV rGPΔmuc without positive control serum
was 0.071 OD650