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Flow Cytometry
Flow Cytometry:
Flow cytometry is a powerful technique used in biological research and clinical diagnostics to analyze and characterize cells based on their physical and chemical properties.
It works by suspending cells in a stream of fluid and passing them through an electronic detection apparatus, typically a laser beam. As cells flow through the laser, they scatter light, and fluorescently labeled cells emit light at specific wavelengths when excited by the laser. This allows for the simultaneous measurement of multiple parameters of individual cells, such as size, granularity, and the presence of specific proteins or nucleic acids.
Flow cytometry is valuable for a wide range of applications, including immunophenotyping, cell cycle analysis, apoptosis detection, and sorting of specific cell populations.
Its high throughput and multiparametric capabilities make it an indispensable tool in many fields of biological and medical research.