Recombinant EBOV GPDmuc (S2)

Product Description

Recombinant EBOV GPDmuc (S2) is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: ELISA, WB

Description: Mature, recombinant, His-tagged Ebola virus Glycoprotein minus the transmembrane domain
(EBOV rGPΔTM) is supplied as purified protein. EBOV rGPΔTM is produced in S2 insect cells and is purified by FPLC.

Storage: 2-3 weeks at -20°C, -80°C long term

Size: 100 µg of protein supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a concentration of 0.615 mg/mL. The theoretical molecular weight of the protein is ~68 kDa including the His-tag, without glycosylatio.

Relevance: Recombinant glycoprotein provides a means as a control protein for immunoassays and a tool to enhance research.

Western Blot: Quality control testing demonstrates strong detection of GP under reduced conditions.

SDS-PAGE & Western Blot Detection

(A) SDS-PAGE and stain demonstrating 5 µg and 1 µg
(lanes 1, 2 respectively) of EBOV rGPΔTM His-tag protein
under denaturing and reducing conditions. MW denotes
Novex® Sharp prestained protein marker. (B) Western
blot detection of EBOV rGPΔTM at 500 ng, 100 ng and 50
ng (lanes 1-3, respectively) using rabbit anti-EBOV GP
polyclonal antibody (catalog# 0301-015) at 0.5 µg/mL
and anti-rabbit IgG-HRP conjugate, followed by TMB
membrane substrate.

ELISA Data

Plate was coated with EBOV rGPΔTM starting at 800
ng/well, serially diluted in DPBS. Washed plate was
detected using one dilution of a positive control serum,
followed with anti-IgG HRP conjugate and TMB
substrate. OD650 is reported. Background of EBOV
rGPΔTM coated plate positive control serum was 0.073
OD650