Recombinant EBOV VP40

Product Description

Recombinant EBOV VP40 is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: ELISA, WB

Description: Recombinant, tag-free, purified Ebola virus (EBOV) matrix protein (VP40) is
expressed in E. coli and the recombinant protein purified using FPLC.

Storage: -80°C. It is recommended to dispense single-use aliquots and store aliquots at -80°C to
avoid multiple freeze/thaw cycles.

Size: 100 µg of protein is supplied in HEPES buffer pH 7.5 containing sodium chloride, 5% glycerol
and 0.1% Triton-X, at a concentration of 1.385 mg/mL. The theoretical molecular weight of the
protein is ~35 kDa.

Relevance: Recombinant EBOV VP40 matrix protein provides a means as a control protein for
immunoassays and a tool to enhance Filovirus research.

SDS-PAGE & Western Blot: Quality control testing shows a prominent protein band below 40 kDa (VP40).

SDS-PAGE & Western Blot Detection:

(A) SDS-PAGE and stain demonstrating 5 ug and 1
ug (lanes 1-2) of EBOV VP40 protein under
denaturing and reducing conditions. MW denotes
Novex® Sharp pre-stained protein markers. (B)
Western blot detection of EBOV VP40 at 50 ng and
100 ng (lanes 1-2). EBOV VP40 was detected using
IBT’s polyclonal antibody at 50 ng/mL (Cat. #
0301-010) and anti-rabbit IgG-HRP conjugate,
followed by TMB membrane substrate.

ELISA Data:

Plate was coated with EBOV VP40 starting at 800
ng/well, serially diluted in DPBS. Washed plate
was detected using one dilution of a positive
control serum, followed with anti-IgG HRP
conjugate and TMB substrate. OD650 is reported.